The group's maintenance and industrial services division will provide maintenance services across three packages of work under a three-year contract, with two possible three-year extension options.
The A$60 million (US$41 million) contract covers general track maintenance and renewals services on the coastal component of the rail network, resurfacing services across the entire rail network, and rail workshop services.
Monadelphous managing director Rob Velletri said the contract built on the company's long-term relationship with Rio Tinto in the delivery of construction and maintenance services, and further broadened Monadelphous' service offering.
"This work will also provide us with the opportunity to strengthen and build on our existing capabilities in the rail sector," he added.
Monadelphous said its current offering of rail services across the Pilbara region included mechanical repair, abrasive blasting, cleaning and relining of carbon steel ore wagons, and the provision of commissioning services for new ore wagons.
Earlier this month, the engineer was awarded work on the expansion of Rio Tinto's West Angelas iron ore mine.